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<i>C. vulgaris</i> has a positive effect on the removal of nutrients from pig farm biogas slurry. However, swine wastewater often contains heavy metal ions, such as Cu (II), which may have impacts on the nutrient removal performance of <i>C. vulgaris</i>. Additionally, the heavy metal ions in wastewater can be adsorbed by microalgae. In this study, the stress effect of Cu (II) on the growth of <i>Chlorella vulgaris</i>, the Cu (II) removal by microalgae, and the effect of different concentrations of Cu (II) on the nutrient removal efficiency of <i>C. vulgaris</i> in biogas slurries were explored. The results showed that the microalgae biomass of microalgae on the sixth day of the experiment was the highest in the treatment with a Cu (II) concentration of 0.5 mg/L, which was 30.1% higher than that of the 2.5 mg/L group. <i>C. vulgaris</i> had higher removal efficiencies of Cu (II) at a Cu (II) concentration of 0.1~1.5 mg/L. The–OH, C=O, –COOH, and C–O groups on the surface of the algal cells play a significant role in the removal of Cu (II). The removal rates of COD, NH₃–N, TN, and TP by <i>C. vulgaris</i> at a Cu (II) concentration of 0.5 mg/L were the highest, which were 89.0%, 53.7%, 69.6%, and 47.3%, respectively.