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This article describes a case of Dictyocaulus arnfieldi infestation in a horse with signs of mild pneumonia in Mashhad zoo. In hematological examinations leukocytosis, neutrophilia with degenerative left shift; and in parasitological examination the first stage larvae of Dictyocaulus arnfieldi were detected in the feces. According to the authors' knowledge, this is the first report of D. arnfieldi infestation in Iran.