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Abstract Background This study aimed to further complete normative data sets for the strength profile of the thigh in soccer players by performing isometric and isokinetic measurements in a large sample per player position. Methods In total, 364 soccer players were divided into subgroups according to their pitch position. All players performed isometric and isokinetic strength measurements during active competition period using an isokinetic dynamometer (System 3, Biodex®, ENRAF-NONIUS, New York, USA). Results Isokinetic strength of m. Quadriceps was significantly (p = 0.023) higher in strikers compared to central midfielders in both the dominant (+ 9%) and the non-dominant (+ 4%) leg. No further differences were found in isometric or isokinetic strength per playing positions. Conclusions We have shown that strength profiles do not differ between playing positions on the field. This is valuable information for elite soccer high performance coaches and medical staffs aiming to improve daily training efficiency and rehabilitation of their players.