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We provide new data on the distribution and biology of the three Stenodynerus species (Hymenoptera, Vespidae, Eumeninae) from Panama: Stenodynerus iolans (Cameron, 1905) is recorded for the first time from Panama; several localities are added to the known distribution in Panama of S. farias (Saussure, 1857); and S. otomitus (Saussure, 1857), currently known as a ground-nester, was found emerging from exposed cells together with Ipsiura pilifrons (Cameron, 1888) (Hymenoptera, Chrysididae).