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An 8-week feeding trial was performed to investigate the effects of dietary bile acids on growth, glucose metabolism, and intestinal health in spotted seabass (<i>Lateolabrax maculatus</i>) reared at high temperatures (33 °C). The fish (20.09 ± 1.12 g) were fed diets supplemented with bile acids: 0 (Con), 400 (BA400), 800 (BA800), and 1200 (BA1200) mg/kg, respectively. The results showed that the growth was promoted in fish at the BA800 treatment compared with the control (<i>p</i> < 0.05). Increased enzyme activities and transcripts of gluconeogenesis in the liver were observed, whereas decreased enzyme activities and transcripts of glycolysis, as well as glycogen content, were shown in the BA800 treatment (<i>p</i> < 0.05). The transcripts of bile acid receptors <i>fxr</i> in the liver were up-regulated in the BA800 treatment (<i>p</i> < 0.05). A bile acid supplementation of 800 mg/kg improved the morphological structure in the intestine. Meanwhile, intestinal antioxidant physiology and activities of lipase and trypsin were enhanced in the BA800 treatment. The transcripts of genes and immunofluorescence intensity related to pro-inflammation cytokines (<i>il-1β</i>, <i>il-8</i>, and <i>tnf-α</i>) were inhibited, while those of genes related to anti-inflammation (<i>il-10</i> and <i>tgf-β</i>) were induced in the BA800 treatment. Furthermore, transcripts of genes related to the NF-κB pathway in the intestine (<i>nfκb</i>, <i>ikkα</i>, <i>ikkβ</i>, and <i>ikbα1</i>) were down-regulated in the BA800 treatment. This study demonstrates that a dietary bile acid supplementation of 800 mg/kg could promote growth, improve glucose metabolism in the liver, and enhance intestinal health by increasing digestive enzyme activity and antioxidant capacity and inhibiting inflammatory response in <i>L. maculatus</i>.