RNA structure probing reveals the structural basis of Dicer binding and cleavage

oleh: Qing-Jun Luo, Jinsong Zhang, Pan Li, Qing Wang, Yue Zhang, Biswajoy Roy-Chaudhuri, Jianpeng Xu, Mark A. Kay, Qiangfeng Cliff Zhang

Format: Article
Diterbitkan: Nature Portfolio 2021-06-01

Deskripsi

Sequencing methods such as icSHAPE were developed to probe RNA structures transcriptome-wide in cells. To probe intact RNA structures, the authors develop icSHAPE-MaP and apply to Dicer-bound substrates showing that distance measuring is important for Dicer cleavage of pre-miRNAs.