Metabolic response induced by endophytic fungi and bacteria in H. marrubioides Epling in vitro microplants
oleh: Luciana Cristina Vitorino, Fabiano Guimarães Silva, William Cardoso Lima, Marcos Antônio Soares, Rita Cássia Nascimento Pedroso, Marolí Rodrigues Silva, Herbert Júnior Dias, Antônio Eduardo Miller Crotti, Márcio Luís Andrade e Silva, Wilson Roberto Cunha, Patrícia Mendonça Pauletti, Ana Helena Januário
| Format: | Article |
|---|---|
| Diterbitkan: | Sociedade Brasileira de Química 2013-01-01 |
Deskripsi
Hyptis marrubioides Epling is a native plant from Brazilian Cerrado. In this paper, the response of in vitro microplants of this species to inoculation with bacterial and fungal endophytic isolates is evaluated. HPLC-DAD analysis showed the presence of 3,4-O-(Z)-dicaffeoylquinic acid and quercetin-7-O-glucoside as the main components. GC/MS analysis demonstrated that the sesquiterpenes τ-cadinol and caryophyllene oxide were only produced in microplants inoculated with endophytic bacteria, while methyl hexadecanoate, methyl heptadecanoate and methyl (Z,Z,Z) 9,12,15-octadecatrienoate and the triterpene methyl 3β-hydroxy-urs-12-en-28-oate were overexpressed only when the microplant was treated with endophytic fungi.