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The protein family with nucleotide binding sites and leucine-rich repeat (NBS-LRR) in plants stimulates immune responses caused by effectors and can mediate resistance to hemi-biotrophs and biotrophs. In our previous study, a Toll-interleukin-1(TIR)-NBS-LRR gene cloned from <i>Vitis amurensis</i> &#8220;Shuanghong&#8221;, <i>VaRGA1</i>, was induced by <i>Plasmopara viticola</i> and could improve the resistance of tobacco to <i>Phytophthora capsici</i>. In this study, <i>VaRGA1</i> in &#8220;Shuanghong&#8221; was also induced by salicylic acid (SA), but inhibited by jasmonic acid (JA). To investigate whether <i>VaRGA1</i> confers broad-spectrum resistance to pathogens, we transferred this gene into <i>Arabidopsis</i> and then treated with <i>Hyaloperonospora arabidopsidis</i> (<i>Hpa</i>), <i>Botrytis cinerea</i> (<i>B. cinerea</i>), and <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000 (<i>Pst</i>DC3000). Results showed that <i>VaRGA1</i> improved transgenic <i>Arabidopsis thaliana</i> resistance to the biotrophic <i>Hpa</i> and hemi-biotrophic <i>Pst</i>DC3000, but decreased resistance to the necrotrophic <i>B. cinerea</i>. Additionally, qPCR assays showed that <i>VaRGA1</i> plays an important role in disease resistance by activating SA and inhibiting JA signaling pathways. A 1104 bp promoter fragment of <i>VaRGA1</i> was cloned and analyzed to further elucidate the mechanism of induction of the gene at the transcriptional level. These results preliminarily confirmed the disease resistance function and signal regulation pathway of <i>VaRGA1</i>, and contributed to the identification of R-genes with broad-spectrum resistance function.