Generation of Gαi knock-out HEK293 cells illuminates Gαi-coupling diversity of GPCRs
oleh: Yuki Ono, Kouki Kawakami, Gaku Nakamura, Satoru Ishida, Junken Aoki, Asuka Inoue
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2023-01-01 |
Deskripsi
A HEK293 cell line lacking all Gαi subunits is generated which enables the measurement of coupling between a GPCR and individual Gαi subunits upon transient re-expression of a specific Gαi subunit.