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<i>Moraxella</i> is an ocular bacterial pathogen isolated in cases of keratitis, conjunctivitis, and endophthalmitis. Gram-negative brick-shaped diplobacilli from ocular specimens, and slow growth in culture, are early indications of <i>Moraxella</i> ocular infection; however, identifying <i>Moraxella</i> to species can be complex and inconsistent. In this study, bacteria consistent with <i>Moraxella</i> were identified to species using: (1) DNA sequencing coupled with vancomycin susceptibility, (2) MALDI-TOF mass spectrometry, and (3) the Biolog ID system. Study samples consisted of nine ATCC <i>Moraxella</i> controls, 82 isolates from keratitis, 21 isolates from conjunctivitis, and 4 isolates from endophthalmitis. The ATCC controls were correctly identified. For keratitis, 66 (80.5%) were identified as <i>M.</i> <i>nonliquefaciens</i>, 7 (9.0%) as <i>M. lacunata</i>, 5 (6%) as <i>M. osloensis</i>, 2 (2.5%) as <i>Acinetobacter lwoffii</i>, 1 (1.0%) as <i>M.</i> <i>bovis/nonliquefaciens</i>, and 1 (1.0%) as <i>M.</i> <i>osloensis/nonliquefaciens</i>. For conjunctivitis, 9 (43.0%) were identified as <i>M.</i> <i>osloensis</i>, 6 (29.0%) as <i>M. nonliquefaciens</i>, 3 (14.3%) as <i>Roseomonas</i>, 2 (9.5%) as <i>Acinetobacter</i> (<i>parvus, junii</i>), and 1 (4.5%) as <i>M. catarrhalis/nonliquefaciens</i>. From endophthalmitis, 3 of 4 of the isolates were <i>M.</i> <i>nonliquefaciens</i>. Overall, <i>M</i>. <i>nonliquefaciens</i> and <i>M.</i> <i>osloensis</i> were identified in 70% (75 of 107) and 13% (14 of 107) of cases, respectively, totaling 83% (89 of 107). <i>M. nonliquefaciens</i> and <i>M.</i> <i>osloensis</i> are important bacterial pathogens of the eye as determined by DNA sequencing, MALDI-TOF MS, and Biolog. Although <i>Moraxella</i> <i>catarrhalis</i> is a clinical pathogen, other species of <i>Moraxella</i> appear to have a prominent role in eye infections.