Evaluations of the Peroxidative Susceptibilities of Cod Liver Oils by a <sup>1</sup>H NMR Analysis Strategy: Peroxidative Resistivity of a Natural Collagenous and Biogenic Amine-Rich Fermented Product

oleh: Benita C. Percival, Angela Wann, Richard Zbasnik, Vicki Schlegel, Mark Edgar, Jie Zhang, Gilbert Ampem, Philippe Wilson, Adam Le Gresley, Declan Naughton, Martin Grootveld

Format: Article
Diterbitkan: MDPI AG 2020-03-01

Deskripsi

High-resolution <sup>1</sup>H nuclear magnetic resonance (NMR) analysis was employed to molecularly screen the lipid, lipid oxidation product (LOP), and antioxidant compositions of four natural (unrefined) cod liver oil (CLO) products. Products 1&#8722;3 were non-fermented CLOs, whilst Product 4 was isolated from pre-fermented cod livers. Supporting analytical data that were acquired included biogenic amine, flavanone, tannin, phenolic antioxidant, &#945;-tocopherol, and oxygen radical absorbance capacity (ORAC) determinations by recommended HPLC, LC/MS/MS, or spectrophotometric methods. SDS-PAGE, HPLC, and <sup>1</sup>H NMR analyses investigated and determined collagenous antioxidants and their molecular mass ranges. <sup>1</sup>H NMR analysis of aldehydic LOPs was employed to explore the susceptibilities/resistivities of each CLO product to peroxidation that is induced by thermal stressing episodes (TSEs) at 180&#176;C, or following prolonged (42 day) storage episodes at 4 and 23 &#176;C. Product 4 displayed extremely high ORAC values, which were much greater than those of Products 1&#8722;3, and that were predominantly ascribable to significant levels of peroxidation-blocking and/or aldehyde-consuming collagenous polypeptides/peptides and ammoniacal agents therein. Significantly lower levels of toxic aldehydes were generated in the pre-fermented Product 4 during exposure to TSEs, or the above long-term storage episodes. These results confirmed the enhanced peroxidative resistivity of a fermented, antioxidant-fortified natural CLO product over those of non-fermented unrefined products. Product 4: Green Pasture Blue Ice&#8482; Fermented Cod Liver Oil.