Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
oleh: Bo Zhang, Diyin Luo, Yu Li, Vanja Perčulija, Jing Chen, Jinying Lin, Yangmiao Ye, Songying Ouyang
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-06-01 |
Deskripsi
Here, structures of the Cas12i1 R-loop complexes before and after target DNA cleavage and biochemical assays offer detailed insights into the mechanisms of target DNA duplex unwinding, R-loop formation and DNA cleavage, and suggest that Cas12i1 forms a 19-bp long DNA-RNA heteroduplex with the crRNA guide region.