Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection
oleh: Cassandra E. Faux, Katherine E. Arden, Stephen B. Lambert, Michael D. Nissen, Terry M. Nolan, Anne B. Chang, Theo P. Sloots, Ian M. Mackay
| Format: | Article |
|---|---|
| Diterbitkan: | Centers for Disease Control and Prevention 2011-02-01 |
Deskripsi
We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.