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Anaplasma bovis is a leukocytotropic agent of bovine anaplasmosis and there is no available information about molecular study on this agent in cattle of Iran. In this study a total 150 cattle blood samples were collected from central part of Iran. The presence of A. bovis examined using light microscopic detection and species-specific nested polymerase chain reaction (nested-PCR) based on 16S rRNA gene. Of the 150 cattle, 4 (2.66 %) was positive for A. bovis by nested-PCR. These data is the first A. bovis DNA presence in cattle from central part of Iran.