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Up-Regulation of S100A8 and S100A9 in Pulmonary Immune Response Induced by a <i>Mycoplasma capricolum</i> subsp. <i>capricolum</i> HN-B Strain
oleh: Zhenxing Zhang, Xiangying Chen, Yong Meng, Junming Jiang, Lili Wu, Taoyu Chen, Haoju Pan, Zizhuo Jiao, Li Du, Churiga Man, Si Chen, Fengyang Wang, Hongyan Gao, Qiaoling Chen
Format: | Article |
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Diterbitkan: | MDPI AG 2024-07-01 |
Deskripsi
<i>Mycoplasma capricolum</i> subsp. <i>capricolum</i> (Mcc), a member of the <i>Mycoplasma mycoides</i> cluster, has a negative impact on the goat-breeding industry. However, little is known about the pathogenic mechanism of Mcc. This study infected mice using a previously isolated strain, Mcc HN-B. Hematoxylin and eosin staining, RNA sequencing, bioinformatic analyses, RT-qPCR, and immunohistochemistry were performed on mouse lung tissues. The results showed that 235 differentially expressed genes (DEGs) were identified. GO and KEGG enrichment analyses suggested that the DEGs were mainly associated with immune response, defensive response to bacteria, NF-kappa B signaling pathway, natural killer cell-mediated cytotoxicity, and T cell receptor signaling pathway. RT-qPCR verified the expression of <i>Ccl5</i>, <i>Cd4</i>, <i>Cd28</i>, <i>Il2rb</i>, <i>Lck</i>, <i>Lat</i>, <i>Ptgs2</i>, <i>S100a8</i>, <i>S100a9</i>, and <i>Il-33</i>. The up-regulation of S100A8 and S100A9 at the protein level was confirmed by immunohistochemistry. Moreover, RT-qPCR assays on Mcc HN-B-infected RAW264.7 cells also showed that the expression of <i>S100a8</i> and <i>S100a9</i> was elevated. S100A8 and S100A9 not only have diagnostic value in Mcc infection but also hold great significance in clarifying the pathogenic mechanism of Mcc. This study preliminarily elucidates the mechanism of Mcc HN-B-induced lung injury and provides a theoretical basis for further research on Mcc–host interactions.