Comparison of Two <i>Cutibacterium acnes</i> Biofilm Models

oleh: Jennifer Varin-Simon, Fabien Lamret, Marius Colin, Sophie C. Gangloff, Céline Mongaret, Fany Reffuveille

Format: Article
Diterbitkan: MDPI AG 2021-09-01

Deskripsi

The study of biofilms in vitro is complex and often limited by technical problems due to simplified models. Here, we compared <i>C. acnes</i> biofilm formation, from species involved in bone and prosthesis infection, in a static model with a dynamic model. Using similar parameters, the percentage of live bacteria within the biofilm was higher in dynamic than in static approach. In both models, bacterial internalization in osteoblast-like cells, playing the role of stress factor, affected this proportion but in opposite ways: increase of live bacteria proportion in the static model (×2.04 ± 0.53) and of dead bacteria proportion (×3.5 ± 1.03) in the dynamic model. This work highlights the huge importance in the selection of a relevant biofilm model in accordance with the environmental or clinical context to effectively improve the understanding of biofilms and the development of better antibiofilm strategies.