Single-cell RNA cap and tail sequencing (scRCAT-seq) reveals subtype-specific isoforms differing in transcript demarcation
oleh: Youjin Hu, Jiawei Zhong, Yuhua Xiao, Zheng Xing, Katherine Sheu, Shuxin Fan, Qin An, Yuanhui Qiu, Yingfeng Zheng, Xialin Liu, Guoping Fan, Yizhi Liu
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-10-01 |
Deskripsi
Most single-cell RNA sequencing methods have limited ability to profile the transcriptome at isoform resolution. Here the authors develop scRCAT-seq to characterize the 5′- and 3′-ends of transcripts in single cells, and identify cell-type specific alternative transcript isoforms.