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In the present work, we report the biosynthesis of Au nanoparticles using fruit extract of Ribes nigrum. First, the synthesis of gold nanoparticles was performed, and then the structural characteristics, antimicrobial activity and cytotoxicity were assessed. The structural properties of biosynthesized gold nanoparticles were characterized by UV–Vis spectroscopy, Atomic Force Microscopy (AFM) measurements, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FTIR). The antimicrobial properties of synthesized Au nanoparticles were tested against standard and clinical strains of bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli), yeast (Candida albicans) and filamentous fungi (Aspergillus niger, Trichophyton rubrum). The conducted studies constitute the basis for further investigations of the potential use of nanogold as chemotherapeutics. Keywords: Biosynthesis, Gold nanoparticles, Antimicrobial activity, Cytostatic activity