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Background & aim: Throughout the world, half of the people over 70 years of age suffer from knee osteoarthritis and detection of the factors associated with this disease is important. The present study was conducted to examine the association of knee joint anatomic parameters with osteoarthritis occurrence. Methods: In the present observational-comparative study, ninety-four patients with joint osteoarthritis as case group and 106 healthy individuals as control group were enrolled from Orthopedic Clinic of Shahrekord Hospital into the study for comparison. Anatomical parameters such as tibial plateau tilt, Cam effect, and tibial plateau coverage by femoral condyles were calculated by using radiographic findings and compared between two groups. The data were analyzed by SPSS software 16 using chi-square and Pearson correlation Results: Tibial plateau tilt values in patients with osteoarthritis of knee (11.9±3.4°) was significantly higher than the control group (10.2±1.9 °) (p<0.001). Cam effect in patients with osteoarthritis (1.8 ± 0.1) was higher than control group (1.7± 0.1) (p< 0.001). Index of tibial plateau articular surface occupied by condyles of femur in patients with osteoarthritis were 2/3±9/51% and 1/2±1/50% in the control group respectively (p0.05). Conclusion: Anatomic parameters under study could be used to predict osteoarthritis occurrence in healthy individuals.