Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos
oleh: Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, Norbert Perrimon
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-11-01 |
Deskripsi
Forward genetic approaches such as CRISPR screens are powerful ways to identify essential genes and those that influence host-pathogen interactions. Here the authors design a bioinformatics portal for sgRNA design and a recombination-mediated cassette system for delivery into mosquito cell lines.