Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH.
oleh: Reelina Basu, Lan-Tian Lai, Zhenyu Meng, Jun Wu, Fangwei Shao, Li-Feng Zhang
| Format: | Article |
|---|---|
| Diterbitkan: | Public Library of Science (PLoS) 2014-01-01 |
Deskripsi
Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and single-molecule level in cellular contexts.