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<i>Thaumatotibia</i> <i>leucotreta</i> is endemic to southern Africa and is highly significant for various fruit industries, including the South African citrus industry, due to its classification as a phytosanitary pest. Mutualistic associations between <i>C</i>. <i>pomonella</i>, closely related to <i>T</i>. <i>leucotreta</i>, and yeasts have previously been described and reported to reduce larval mortality and enhance larval development. Here, we determined which yeast species occur naturally in the gut of <i>T</i>. <i>leucotreta</i> larvae and investigated whether any of the isolated yeast species affect their behaviour and development. Navel oranges infested with <i>T</i>. <i>leucotreta</i> larvae were collected from geographically distinct provinces in South Africa, and the larvae were processed for analysis of naturally occurring associated yeasts. Six yeast species were isolated and identified from the guts of these <i>T</i>. <i>leucotreta</i> larvae via PCR amplification and sequencing of the ITS region of rDNA and D1/D2 domain of large ribosomal subunit. Larval development and attraction assays were conducted, and <i>T</i>. <i>leucotreta</i> larvae that fed on Navel oranges inoculated with yeast had accelerated developmental periods and reduced mortality rates. Neonate <i>T. leucotreta</i> were also attracted to YPD broth cultures inoculated with yeast for feeding. Oviposition preference assays were conducted with adult <i>T</i>. <i>leucotreta</i> females. Navel oranges inoculated with yeast were shown to influence the oviposition preference of adult females. Yeasts harbour the potential for use in biocontrol, especially when combined with other well-established control methods. This study provides a platform for future research into incorporating yeast with current biological control agents as a novel option for controlling <i>T</i>. <i>leucotreta</i> in the field.