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A high-density linkage map of the <it>RN </it>region in pigs
oleh: Chardon Patrick, Kalm Ernst, Robic Annie, Amarger Valérie, Rey Virginie, Rogel-Gaillard Claire, Reinsch Norbert, Paul Sven, Jeon Jin-Tae, Milan Denis, Looft Christian, Andersson Leif
Format: | Article |
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Diterbitkan: | BMC 2000-05-01 |
Deskripsi
<p>Abstract</p> <p>The porcine <it>RN </it>locus affects muscle glycogen content and meat quality. We previously mapped the <it>RN </it>locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the <it>RN </it>region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order <it>FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906</it>. The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the <it>RN </it>gene.</p>