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Genotyping on ALDH2: comparison of four different technologies.
oleh: Lina Zhang, Jinzhao Zhao, Guanglin Cui, Hong Wang, Dao Wen Wang
Format: | Article |
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Diterbitkan: | Public Library of Science (PLoS) 2015-01-01 |
Deskripsi
<h4>Objectives</h4>This study aimed to compare the accuracy and performance of four genotyping methods for detecting single nucleotide polymorphisms (SNPs) in aldehyde dehydrogenase-2 (ALDH2), which is the principal enzyme involved in alcohol metabolism.<h4>Design and methods</h4>We genotyped rs671 of ALDH2 in 96 coronary heart disease (CHD) patients with four methods including high resolution melting analysis (HRM), TaqMan allelic discrimination assay (TaqMan), allele-specific PCR (AS-PCR) and pyrosequencing. Meanwhile, we compared the accuracy and performance of these methods.<h4>Results</h4>All selected patients were successfully genotyped with referred methods. The results of these four assays showed 100% concordant results and had 100% accuracy as verified by Sanger sequencing.<h4>Conclusions</h4>All of the referred methods can be used for genotyping ALDH2 rs671 with the same accuracy compared to Sanger sequencing. In small size of clinical samples, HRM and AS-PCR outperform over others due to their lower cost and less hands-on operation, which are suitable for clinical application.