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Vector-like quarks (VLQs) are predicted in many new physics scenarios beyond the standard model (SM). We consider a search strategy for vector-like bottom quark (VLQ-B) at the LHC in a model independent fashion. Our analysis is based on single production of VLQ-B with decaying to the Zb mode. The production cross sections and the signal significance SS are calculated, and the observability of the VLQ-B signal is investigated. Our numerical results show that the possible signals of the VLQ-B with electric charge of QB=−1/3, which is the SU(2) singlet, might be detected via the process pp→B→Z(→l+l−)b at the upgraded LHC.