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Quality Assurance (QA) for medical linear accelerator (linac) is one of the primary concerns in external beam radiation Therapy. Continued advancements in clinical accelerators and computer control technology make the QA procedures more complex and time consuming which often, adequate software accompanied with specific phantoms is required. To ameliorate that matter, we introduce QALMA (Quality Assurance for Linac with MATLAB), a MALAB toolkit which aims to simplify the quantitative analysis of QA for linac which includes Star-Shot analysis, Picket Fence test, Winston–Lutz test, Multileaf Collimator (MLC) log file analysis and verification of light & radiation field coincidence test. Keywords: Star shot, Picket fence, Winston–Lutz, Light radiation coincidence, Log file analysis, Quality assurance