Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi
oleh: Gisele Fernanda Assine Picchi-Constante, Priscila Mazzocchi Hiraiwa, Martin Marek, Vanessa Zulkievicz Rogerio, Eloise Pavão Guerra-Slompo, Christophe Romier, Nilson Ivo Tonin Zanchin
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2022-06-01 |
Deskripsi
Summary: This protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We combine CRISPR-Cas9 technology with recombination of variants of the target gene containing the desired mutations that are resistant to Cas9-cleavage, which enables detailed investigation of protein function. This experimental strategy overcomes some of the limitations associated with gene knockouts in T. cruzi.For complete details on the use and execution of this protocol, please refer to Marek et al. (2021).