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Equine-facilitated interventions have shown promise for facilitating emotional and behavioural changes in diverse groups. The current study evaluated the effectiveness of an equine workshop for vulnerable Guatemalan youth using a mixed-method approach. The 37 participants (Mage = 18.22, SD = 2.25, 14 girls) came from difficult circumstances including poverty and other risks. Using a wait-list control group design with random assignment, the effects of a 2-day equine-based workshop were evaluated. Participants completed quantitative measures of leadership, emotion regulation, aggression, and interpersonal response to threat. Mentors completed reports of aggression and prosocial behaviour. Self-reported leadership increased significantly in the group receiving the intervention; mentor reports of aggression revealed significant decreases. Focus groups with participants and family members reported multiple benefits, including improved emotion regulation. Equine-based interventions may provide at-risk Guatemalan youth with tools of leadership, reduced violence and aggression, and better emotion regulation.