Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment
oleh: Bing-Rui Zhou, K. N. Sathish Yadav, Mario Borgnia, Jingjun Hong, Baohua Cao, Ada L. Olins, Donald E. Olins, Yawen Bai, Ping Zhang
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-05-01 |
Deskripsi
CENP-A histone variants replace histones H3 at centromeres. Here the authors use a single-chain antibody fragment (scFv) to stabilize human CENP-A nucleosome containing a native α-satellite DNA and solved its structure by cryo-EM to 2.6 Å resolution, providing insight into the structure and function of the CENP-A nucleosome.