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Rice is one of the most important crops in the world, and its production is severely affected by the rice blast disease caused by the fungus <i>Magnaporthe oryzae</i>. Several major blast resistance genes and QTLs associated with blast resistance have been described and mostly identified in <i>indica</i> rice varieties. In this work, we report the obtention of a blast-resistant rice breeding line derived from crosses between the resistant <i>indica</i> variety CT13432 and the <i>japonica</i> elite cultivar JSendra (highly susceptible to blast). The breeding line, named COPSEMAR9, was found to exhibit resistance to leaf blast and panicle blast, as demonstrated by disease assays under controlled and field conditions. Furthermore, a high-quality genome sequence of the blast-resistant breeding line was obtained using a strategy that combines short-read sequencing (Illumina sequencing) and long-read sequencing (Pacbio sequencing). The use of a whole-genome approach allowed the fine mapping of DNA regions of <i>indica</i> and <i>japonica</i> origin present in the COPSEMAR9 genome and the identification of parental gene regions potentially contributing to blast resistance in the breeding line. Rice blast resistance genes (including <i>Pi33</i> derived from the resistant parent) and defense-related genes in the genome of COPSEMAR9 were identified. Whole-genome analyses also revealed the presence of microRNAs (miRNAs) with a known function in the rice response to <i>M. oryzae</i> infection in COPSEMAR9, which might also contribute to its phenotype of blast resistance. From this study, the genomic information and analysis methods provide valuable knowledge that will be useful in breeding programs for blast resistance in <i>japonica</i> rice cultivars.