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In this paper, aluminium oxide nanoparticles (ANPs) were added to Mahua biodiesel blend (MME20) in different proportions to investigate the effects on a four stroke, single cylinder, common rail direct injection (CRDI) diesel engine. The ANPs were doped in different proportions with the Mahua biodiesel blend (MME20) using an ultrasonicator and a homogenizer with cetyl trimethyl ammonium bromide (CTAB) as the cationic surfactant. The experiments were conducted in a CRDI diesel engine at a constant speed of 1500 rpm using different ANP-blended biodiesel fuel (MME20 + ANP50 and MME20 + ANP100) and the results were compared with those of neat diesel and Mahua biodiesel blend (MME20). The experimental results exposed a substantial enhancement in the brake thermal efficiency and a marginal reduction in the harmful pollutants (such as CO, HC and smoke) for the nanoparticles blended biodiesel.