Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing
oleh: Feima Wu, Ge Gao, Tingcai Pan, Zhen Yang, Guosheng Xu, Nasir Abbas, Yanli Liu, Yan Chen, Shenglin Tan, Kai You, Xinrong Ke, Yuanqi Zhuang, Xianhua Lin, Fan Yang, Yin-xiong Li
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2018-03-01 |
Deskripsi
The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40 bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers.