Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
oleh: Wenlei Bao, Jianxin Yin, Yan Liang, Zhixin Guo, Yanfeng Wang, Dongjun Liu, Xiao Wang, Zhigang Wang
| Format: | Article |
|---|---|
| Diterbitkan: | Asian-Australasian Association of Animal Production Societies 2014-09-01 |
Deskripsi
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.