Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction
oleh: Yijie Guo, Tomokazu Fukuda, Shuichi Nakamura, Lanlan Bai, Jun Xu, Kengo Kuroda, Rintaro Tomioka, Hiroshi Yoneyama, Emiko Isogai
| Format: | Article |
|---|---|
| Diterbitkan: | Asian-Australasian Association of Animal Production Societies 2015-02-01 |
Deskripsi
Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.