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An investigation was conducted on primary productivity and water quality of Sahastradhra hill-stream for evaluation of  aquatic  ecosystem status  in different  environmental settings  along  with  the stream.  The factors such as temperature, pH, carbon dioxide and  plankton  on  which  primary productivity depends  were  studied. It was pointed out that the Gross primary productivity showed a negative relationship with temperature, pH and carbon dioxide. The present paper deals with the production efficiency of Sahastradhara hill stream, Dehradun and maximum production  efficiency of  Sahastradhara  stream was found  66.66% at  sampling  site-  III  in  the  month of December 2003, while minimum production efficiency was recorded 20.79% at site- IV during May 2004.