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Background: This study aimed to develop and practically use a questionnaire to evaluate knowledge, attitude, and practice (KAP) of women regarding the use of sanitizers at home against coronavirus disease 2019 (COVID-19). Methods: An online cross-sectional study was conducted among Iranian women (aged ≥18 years). The KAP items were selected based on the experts’ opinions, and the scale underwent a series of validation processes, including the face, content, and construct validity, and internal consistency for reliability. Results: The internal consistency coefficient exceeded 0.7 for KAP subunits. Exploratory factor analysis (EFA) suggested a three-factor construct for each subunit, and the results of the confirmatory factor analysis (CFA) indicated acceptable fit indices for the proposed models. Overall, 330 women (mean age: 36.78 ± 10.12 years, married: 74.2%, and bachelor’s degree: 46.7%) completed the questionnaire. The level of adequate knowledge on sanitizer use, positive attitude, and good practice achieved were 87.0%, 58.5%, and 66.1%, respectively. Among demographic variables, education level and occupation showed a significant relationship (P < 0.05) against KAP and attitude, individually. Conclusion: Despite the high percentage of knowledge, the participants did not get a high attitude and practice score.