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In this study a total of 69 heifers in 8- 11 months of age; 21 Holstein (H), 17 Southern Anatolian Reds (GAK), 18 Holstein x GAK first cross- bred (G₁) and 13 second cross-bred (G₂) obtained from the Mercimek Zootechnical Research Institute of General Directorate for Agricultural affairs, were used.<p> The experiments have continued for 4 seasons. Therefore a total of 276 blood samples: 4 times from each animal in every season, were collected and 1104 analyses: GOT, BUN, ALP- FF A for each samples were carried out.<p> The analysis of glutamic oxaloacetic transaminase and alkaline phosphatase eİızymes in blood serum were executed by using Boehringer enzyme test kits. For the determination of nitragen in b:ood urea, Nesslerisation method was used. Free fatty acidsin plasma were measured by coloİ'imetric methods. The results were statistically evaluated by analysis of variance and Duncan's multiple range test.<p> According to the seasonal differencies the following values were obtained. The GOT values; 41.44 ±3.84- 82.38 ±5.69 U/L. The ALP values; 36.85 ±2.19 - 110.76 ±7.86 U/L. The BUN values; 10.02 ±0.667- 17.52 ±1.328 mg/100 ml. The FFA values; 0.047 ±0.003 - 0.461 ±0.093 mM/L.<p> The differences related to the seasons and animal breeds are found to be statistically significant (P <0.01, P <0.05).