Triphenylphosphonium conjugated gold nanotriangles impact Pi3K/AKT pathway in breast cancer cells: a photodynamic therapy approach

oleh: Nadar Manimaran Vinita, Umapathy Devan, Sabapathi Durgadevi, Selvaraj Anitha, Dhamodharan Prabhu, Sundarraj Rajamanikandan, Muthusamy Govarthanan, Ananthanarayanan Yuvaraj, Muniyandi Biruntha, Arockiam Antony Joseph Velanganni, Jeyaraman Jeyakanthan, Pitchan Arul Prakash, Mohamed Sultan Mohamed Jaabir, Ponnuchamy Kumar

Format: Article
Diterbitkan: Nature Portfolio 2023-02-01

Deskripsi

Abstract Although gold nanoparticles based photodynamic therapy (PDT) were reported to improve efficacy and specificity, the impact of surface charge in targeting cancer is still a challenge. Herein, we report gold nanotriangles (AuNTs) tuned with anionic and cationic surface charge conjugating triphenylphosphonium (TPP) targeting breast cancer cells with 5-aminoleuvinic acid (5-ALA) based PDT, in vitro. Optimized surface charge of AuNTs with and without TPP kill breast cancer cells. By combining, 5-ALA and PDT, the surface charge augmented AuNTs deliver improved cellular toxicity as revealed by MTT, fluorescent probes and flow cytometry. Further, the 5-ALA and PDT treatment in the presence of AuNTs impairs cell survival Pi3K/AKT signaling pathway causing mitochondrial dependent apoptosis. The cumulative findings demonstrate that, cationic AuNTs with TPP excel selective targeting of breast cancer cells in the presence of 5-ALA and PDT.