B1 SINE-binding ZFP266 impedes mouse iPSC generation through suppression of chromatin opening mediated by reprogramming factors
oleh: Daniel F. Kaemena, Masahito Yoshihara, Meryam Beniazza, James Ashmore, Suling Zhao, Mårten Bertenstam, Victor Olariu, Shintaro Katayama, Keisuke Okita, Simon R. Tomlinson, Kosuke Yusa, Keisuke Kaji
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2023-01-01 |
Deskripsi
Induced pluripotent stem cell (iPSC) reprogramming is inherently inefficient. Here the authors identify 24 reprogramming roadblock genes through a CRISPR/Cas9-mediated genome-wide knockout screen including a KRAB-ZFP Zfp266, knockout of which consistently enhances murine iPSC generation.