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Molecular diversity of the seven wheat (Triticum aestivum L.) genotypes was evaluated using 50 SSR primers. Genetic distances were calculated using unweighted pair group of arithmetic mean (UPGMA) procedure. A high degree of genetic polymorphism was observed among the wheat varieties with average genetic polymorphism 70%. SSR diversity data was generated using above primers and a total of 114 alleles were detected with an average of 2.71 alleles per locus. The number of alleles per locus ranged from 2 to 6 and the percent of polymorphism ranged from 20% for the Xwmc254 to 100% for the Xbarc26. The average genetic diversity based on SSR markers was 0.465 with a range of 0.037- 0.892. Our results suggested that the classification based on genotypic markers of these wheat genotypes would be useful for wheat breeders to plan crosses for positive traits. The results obtained suggested that the wheat microsatellite markers could be used to distinguish wheat genotypes and to estimate genetic diversity. The present study also indicates that microsatellite markers permit the fast and high throughout fingerprinting of accessions from a varieties collection in order to assess genetic diversity.