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Confocal microscopy enabled the identification of store-operated Ca2+ entry in the sec­retory cells of Drosophila melanogaster larval salivary glands, which expresses Ca2+ sensor G-CaMP1.6. To release Са2+ from intracellular stores, we used Са2+ ionophore ionomycin and endoplasmic reticulum Са2+-pump inhibitor thapsigargin. Addition of these compounds to the Са2+-free medium caused slight and non-transient elevation of the cytosolic Са2+ concentration in the secretory cells. After an increase of Са2+ concentration at 2 mmol/l, a significant increase in cytosolic Са2+ level appeared in both cases. Although under the use of ionomycin, an increase of cytosolic Са2+ was transient in all cases, Саe2+-induced Са2+-transients from different cells of investigated gland’s region were synchronous, and determined by activation of store-operated Са2+ entry as well as by its entry from extracellular medium under the ionophoretic exchange. In case of emptying stores by thapsigargin, an increase of extracellular Са2+ concentration induced in part of the cells, fast transients of cytosolic Са2+, in some part – slow primary transients, which changes by non-transient elevation of cytosolic Са2+ level, and in another part – only non-transient increase of cytosolic Са2+ level. Such differen­ces of Саe2+-induced Са2+-transients in different cells is determined, perhaps, by the fact that their generation involved only store-operated Са2+-channels as far as other Са2+-transport systems of plasma membrane and intracellular organelles.