The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in <i>Tilia miqueliana</i> Maxim. Seeds

oleh: Fenghou Shi, Yunxiang Cao, Yajun Gao, Yuhou Qiu, Yizeng Lu, Biao Han, Yongbao Shen

Format: Article
Diterbitkan: MDPI AG 2024-02-01

Deskripsi

The seeds of <i>Tilia miqueliana</i> Maxim. exhibit deep dormancy, which is categorized as combinational dormancy. This study utilized a comprehensive treatment involving magnetic fields, gibberellin (GA<sub>3</sub>), and cold stratification to promote the release of seed physiological dormancy and enhance germination rates. After being soaked in 98% H<sub>2</sub>SO<sub>4</sub> for 15 min, mature seeds of <i>Tilia</i> were exposed to magnetic field treatments (150 MT, 250 MT) for different durations (25 min, 45 min, 65 min, and 85 min), as well as GA<sub>3</sub> solution soaking (concentration: 0 μmol·L<sup>−1</sup>, 1443 μmol·L<sup>−1</sup>). Subsequently, cold stratification (0–5 °C) was applied to investigate the effects of these treatments on seed dormancy release and nutrient transformation. The results indicated that the comprehensive treatment involving magnetic field, GA<sub>3</sub> solution soaking, and cold stratification effectively released the physiological dormancy of <i>Tilia</i> seeds and improved germination rates. Among the treatments, M<sub>150</sub>T<sub>85</sub>G<sub>1443</sub> (magnetic field intensity: 150 MT, magnetic field treatment time: 85 min, GA<sub>3</sub> soaking concentration: 1433 μmol·L<sup>−1</sup>) exhibited the most favorable outcome. After 75 days of cold stratification following the comprehensive treatments, the germination rate of M<sub>150</sub>T<sub>85</sub>G<sub>1443</sub> seeds reached 89%. Additionally, the levels of storage substances such as starches and crude fats within the seeds decreased, while the utilization of soluble sugars and soluble proteins increased. The M<sub>150</sub>T<sub>85</sub>G<sub>1443</sub> treatment exhibited the highest degree of variation, leading to gradual increases in metabolic activities of the seeds and a transition from dormancy to germination.