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Objectives: Assessment of nutritional habits and associations with cardio-metabolic risk factors in four age groups of women partici-pating in the Prevention Education Program, Family Heart Study. Methods: Anthropometric variables, systolic and diastolic blood pressures (SBP, DBP), lipoproteins, glucose and insulin were measured in 141 children, 211 adolescents, 151 women <55 years and 150 women ≥ 55 years. Nutritional data were assessed by 7 days weighted dietary records. For statistics, SPSS 15.0 was used; associations were calculated by multiple logistic regression; p<0.05 was considered significant. Results: The prevalence of CVD risk factors was similar in chil-dren and adolescents except for hypertriglyceridemia which was >3 times more common in adolescents. Thirty six percent of junior women were overweight (BMI ≥25 kg/m2) and 21% had central adiposity obese. Sixty eight year-old women had a far more adverse risk profile than 35 year-old women. In terms of energy consumption, 14 year-old women had the lowest fat intake and the highest consumption of carbohydrates whereas intake of pro-tein was lowest in 10 year-old girls. Intake of unsaturated fat was lower in youths than in adults amounting to 37 g unsaturated fat respectively 53.4% of total fat consumption. The association be-tween energy consumption and overweight was significant and calorie intake was associated with clustering of ≥3 cardiovascular risk factors (OR: 4.72; 95% CI 1.22-18.33). Conclusions: The prevalence of CVD risk factors increased con-tinuously from girls and adolescents to junior and senior women. However, dietary intake was different in the four age groups. Ca-loric intake was associated with overweight and clustering of risk factors in adult women.