Pause sequences facilitate entry into long-lived paused states by reducing RNA polymerase transcription rates
oleh: Ronen Gabizon, Antony Lee, Hanif Vahedian-Movahed, Richard H. Ebright, Carlos J. Bustamante
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-07-01 |
Deskripsi
Transcription elongation by RNA polymerase (RNAP) is interspersed with sequence-dependent pausing which is difficult to study due to spatiotemporal limitations of available methods. Here authors use a high-resolution optical tweezers assay and find that pause sites modify the dynamics of nearly all RNAP molecules.