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The Hestina assimilis belongs to Nymphalidae in Lepidoptera. We described the complete mitogenome of H. assimilis, which is typical circular duplex molecules and 15,262 bp in length, containing the standard metazoan set of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and an A + T-rich region with macro-repeat sequences. All the inferred tRNA secondary structures show the common cloverleaf pattern, with the exception of trnS1(AGN) which lacks the DHU arm. The H. assimilis mitochondrial genome has the same gene order as other lepidopterans.