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The present work experimentally demonstrates a multimode fiber optic sensing setup for total fat detection in raw milk samples. The optical fiber arrangement incorporates a low-coherence Fabry&#8722;Perot cavity operating in dual response. The system provides a phase modulation for a total fat range from 0.97 to 4.36%. Here, the protein remains constant at &#8776;3%. The data indicate that maximum sensitivity close to 616 pm/%fat could be achieved at optimal wavelength operation (500 nm). In addition, the system presented a minimal repeatability error measurement of 0.08%, cross-sensitivity between protein and fat of 0.134, and a regression coefficient of <inline-formula> <math display="inline"> <semantics> <mrow> <msup> <mi>r</mi> <mn>2</mn> </msup> <mo>=</mo> <mn>0.9763</mn> </mrow> </semantics> </math> </inline-formula>. A thermal analysis was also performed, which indicate the temperature immunity of the system. The proposed method represents a low-cost alternative to detect minimal fat variations in raw cow milk.