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Thirty faecal samples were collected from diarrhoeic neonatal calves reared in organized farms and households of Wayanad district. The collected rectal swabs were subjected to bacterial isolation and biochemical tests. Based on their cultural and biochemical characteristics isolates were confirmed as Escherichia coli. Based on clinical signs and bacterial isolation calves were diagnosed as affected with colibacillosis. Antibiotic sensitivity patterns of the isolates were evaluated using standard disc diffusion method in Muller Hinton Agar. Antibiogram revealed susceptibility to Chloramphenicol (70.0 per cent), Trimethoprim/Sulphamethoxazole (60.0 per cent), Ceftriaxone (33.3 per cent), Ampicillin (33.3 per cent) and Tetracycline (3.3 per cent). The sensitivity patterns of the isolates to the three antibiotics viz. Trimethoprim/Sulphamethoxazole, Ceftriaxone and Ampicillin correlated well with clinical response.