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The research about biodiesel produced from cow’s fat using sodium hydroxide (NaOH) as catalyst has been done.  Biodiesel produced from cow’s fat was done through transesterification process using methanol 1:12 which priory react with 0.3% NaOH as catalyst due to oil and methanol weight.  The transesterification process was conducted for 5 hours at 65°C.  Biodiesel from cow’s fat is characterisized by GC-MS and ASTM (American Society for Testing and Materials) method.  Methyl ester which produced from cow’s fat are 31.67% of stearic acid, 25.67% of palmitate acid, and 22.68% of oleate acid.  Biodisel from cow’s fat are qualified as diesel fuel and biodiesel characterisized by ASTM for specific density (0.8705 kg/m3), kinematic viscosity (4,481 mm2/s), flash point (176,5 0C), pour point (21 0C), and Conradson’s carbon residu (0.0139% wt).