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This work aimed to promote in vitro seed germination and disinfestation of the sweet cactus. Seeds were submerged in alcohol at 70% for 1 min. and then treated with sodium hypochlorite solution at different concentrations: 0.0; 0.5; 1.0 and 1.5%. After thistreatment, the seeds were washed four times in distillated, deionized and autoclaved water. Seeds were inoculated in MS medium with different concentrations of sucrose (0.0, 2.5, 5.0, 7.5 and 10.0 g L-1). The experimental design was completely randomized in a factorialexperiment 4 x 5, with five replicates for treatments. Contamination, germination and seedling growth were evaluated. The results of the analysis of variance indicate that there were no significant interactions among factors. There was no significance for sucroseconcentrations on contamination but these treatments showed significant differences for seed germination and seedling growth (p . 0.01). At higher sucrose concentrations there was lower germination and smaller seedlings. There were significant differences betweenthe treatments with sodium hypochlorite for all traits analysed (p . 0.01). There was low contamination with the increase in sodium hypochlorite concentrations with higher germination percentage and taller seedlings.<br><br>Este trabalho objetivou desinfestar e promover a germinacao de sementes de palma doce gin vitroh. As sementes foram mergulhadas em alcool a 70% por 1 min. e, posteriormente, imersas em solucao de hipoclorito de sodio nas concentracoes de 0,0; 0,5; 1,0 e 1,5% com tres gotas de Tween 20 por 10 min. e lavadas em quatro aguas bidestiladas estereis. As sementes foram cultivadas em meio MS suplementado com 0,0; 2,5; 5,0; 7,5 e 10,0% de sacarose. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 4 x 5 (quatro concentracoes de hipoclorito de sodio e cincoconcentracoes de sacarose), com cinco repeticoes. Avaliou-se a contaminacao, germinacao e crescimento da plantula. Nao houve interacao significativa entre os fatores, havendo porem, diferencas significativas entre os tratamentos de hipoclorito e concentracoes de sacarose (p . 0,01). As concentracoes de sacarose nao tiveram efeito significativo na contaminacao do meio, porem com o aumento dos teores de sacarose houve tendencia ao decrescimo na germinacao e crescimento da plantula (p . 0,01). Quanto maior a concentracao de hipoclorito no meio menor foi a contaminacao e maior a germinacao e comprimento daplantula (p . 0,01).