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In this study a new species of <i>Gonolobus</i>, <i>G. villasenorii</i>, is described. It is endemic to Sinaloa, Mexico, distributed in the Sierra Madre Occidental in pine–oak forest. This species is characterized by the presence of lanceolate sepals, margin of the corolla lobes with a whitish-green callus, without being cristate, and anthers with rectangular dorsal appendages. The new species is compared to <i>Gonolobus chloranthus</i> and <i>Gonolobus erianthus</i>. <i>Gonolobus grandiflorus</i>, <i>Gonolobus incerianus</i>, and <i>Gonolobus lozadae</i> are species with which it shares reticulate corollas and flowers more than 2 cm in diameter. A detailed description of the new species includes data on its habitat, ecology, phenology, conservation status, a distribution map, and a section where the new species is compared with similar species and how to recognize it.